Novel Techniques in Microscopy

12 April 2021 – 16 April 2021 OSA Virtual Event - Pacific Time (US & Canada) (UTC - 08:00)

Advances in optical microscopy are continually enhancing imaging performance and versatility. Examples include increasing depth penetration in scattering media, improving resolution beyond the diffraction limit, increasing speed, enhancing sensitivity and/or specificity, developing novel contrast mechanisms, addressing challenges related to intravital imaging, and more.

 


Speakers

  • Sophie Brasselet, Institut FRESNELFrance
    Super Resolution Imaging of Proteins’ Organization in 3D Using Polarized Single Molecule Localization
  • Michelle Digman, University of California IrvineUnited States
  • Farzad Fereidouni, University of California DavisUnited States
  • Reto Fiolka, UT SouthwesternUnited States
  • Liang Gao, University of California Los AngelesUnited States
    High-speed Compressed-sensing Fluorescence Lifetime Imaging Microscopy of Live Cells
  • Roarke Horstmeyer, Duke UniversityUnited States
    Title to Be Announced
  • Fang Huang, Purdue UniversityUnited States
    Ultra-high Resolution Imaging in Whole-cell and Tissue Specimens
  • Clemens Kaminski, University of CambridgeUnited Kingdom
    Visualising Organelle Dynamics at High Speed with Structured Illumination Microscopy
  • Yang Liu, University of PittsburghUnited States
    Bring the Power of Super-resolution Microscopy to Visualize Pathological Changes of Chromatin Architecture in Carcinogenesis
  • Pieter Vanden Berghe, KU LeuvenBelgium
    Second Harmonic Imaging of Microtubules in Neuronal Processes
  • Giuseppe Vicidomini, Istituto Italiano di TecnologiaItaly
    Fluorescence Laser-Scanning Microscopy with SPAD Array Detector: Towards Single-Photon Microscopy
  • Alex Walsh, Texas A&M; UniversityUnited States
    Quantitative Analysis of Autofluorescence Lifetime Images to Determine Cell Function
  • Ji Yi, Johns Hopkins UniversityUnited States

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Committee

  • Kyle Quinn, University of Arkansas, United StatesChair
  • Marie-Claire Schanne-Klein, LOB - Ecole Polytechnique, CNRS, Inserm, FranceChair
  • Nicholas Durr, Johns Hopkins University, United StatesProgram Chair
  • Daniel Elson, Imperial College London, United KingdomProgram Chair
  • Emmanuel Beaurepaire, CNRS / Ecole Polytechnique, France
  • Martin Booth, University of Oxford, United Kingdom
  • Kevin Eliceiri, University of Wisconsin, United States
  • Alexander Jesacher, Innsbruck Medical University, Austria
  • Gail McConnell, University of Strathclyde, United Kingdom
  • Monika Ritsch-Marte, Innsbruck Medical University, Austria
  • Paco Robles, Georgia Institute of Technology, United States
  • Loic Royer, CZ Biohub, United States
  • Klaus Suhling, Univ of London King's College London, United Kingdom
  • Shuo Tang, University of British Columbia, Canada

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Plenary Session

Sandrine Lévêque-Fort

Paris Saclay University

Alternative Strategies for 3D Single Molecule Localization Microscopy

I will present our recent work on 3D imaging in Single Molecule Localization Microscopy, where intrinsic properties of fluorescence emission can be directly used to enhance the resolution.

About the Speaker

Sandrine Lévêque-Fort is a CNRS Researcher Director at the Institute of molecular science (ISMO) in Paris Saclay University. She obtained her PhD on the development of a new acousto-optic imaging approach for imaging through scattering media in the Optical Lab of ESPCI in Paris. She then became a postdoctoral fellow in the physics department of Imperial College, where she started to develop time resolved fluorescence microscopy but also structured illumination strategy. She joined the CNRS in 2001 to develop different strategies to improve spatial and temporal resolution for fluorescence microscopy, by implementing new configurations or used plasmonics substrates to engineered fluorescence emission. Since 2009, she has proposed various approaches to take advantage of supercritical angle fluorescence (SAF) emission as an alternative intrinsic tool given by the fluorophore itself to access axial information. In wide field microscopy this allows a dual depth imaging without any photon loss while preserving ideal sectioning for membrane imaging. In combination with super-resolution microscopy techniques (DONALD/DAISY), these new approaches permit to reveal quantitatively the 3D cellular nanoarchitecture. Since 2016, she has combined structured excitation with single molecule localization. By introducing a time signature within the localization process, this technique called ModLoc permits to retrieve the fluorophores’ information thanks to the phase of their modulated emission and benefits of an enhanced and uniform localization precision.

Dan Oron

Weizmann Institute of Science

Quantum Enhanced Superresolution Confocal Microscopy

We show how the resolution of a standard confocal can be increased fourfold with a twofold axial resolution increase by harnessing the quantum phenomenon of fluorescence antibunching and by its classical analog of fluorescence intermittency.

About the Speaker

Dan Oron earned a B.Sc. in mathematics and physics from the Hebrew university in 1994. He earned his M.Sc. degree in physics from Ben-Gurion University of the Negev in 1998 and received his Ph.D., also in physics, from the Weizmann Institute of Science in 2005, under the guidance of Prof. Yaron Silberberg. After conducting postgraduate research with Prof. Uri Banin at the Hebrew University for two years, he joined the staff of the Weizmann Institute in April 2007. He is currently a professor at the department of Molecular Chemistry and Materials Science at the Weizmann institute. His main research interests are at the interface between light and the nanoscale, studying both the interaction of light with nanostructured materials (mostly inorganic and hybrid semiconductor nanocrystals), optical superresolution methods harnessing both quantum and classical fluctuations in light emission and the optics of biological nanostructured materials.

R. Clay Reid

The Allen Institute for Brain Science

Petascale Microscopy for Brain Mapping: Electron and Light Microscopic Approaches to Connectomics

The reconstruction of neural pathways and connections (connectomics) requires high-resolution microscopy over large volumes, thus requiring extremely large data sets. I’ll discuss approaches, from data collection through segmentation, for analyzing neural circuits at the petascale.

About the Speaker

Clay Reid is Senior Investigator at the Allen Institute for Brain Science, where he started a department in 2012 to study how information is encoded and processed in neural networks of the visual system. Prior to joining the Allen Institute, Reid was Professor of Neurobiology at Harvard Medical School. Throughout his career, he has used a combination of imaging and anatomical approaches to investigate how the structure of neural connections relates to the function of cortical circuits. He has helped to pioneer new methods for recording increasingly large ensembles of neurons to study sensory processing. In parallel, he has developed methods to analyze connections in these ensembles using large-scale anatomical reconstructions (connectomics) with serial-section electron microscopy.

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Special Events

Meet the Plenary Speaker Series

The Biophotonics Congress: Optics in the Life Sciences will feature 3 Plenary Speakers. Following technical talks, join your colleagues for a meet-and-greet and discussion with our Congress Plenary Speakers.

  • Tuesday, 13 April: Dan Oron, Weizmann Institute of Science

  • Wednesday, 14 April: Sandrine Lévêque-Fort, Paris Saclay University

  • Thursday, 15 April: R. Clay Reid, The Allen Institute for Brain Science

Monday, 12 April

Successfully Navigate an OSA Virtual Meeting

The post-COVID world has new challenges in regards to virtual meetings – are you prepared? Listen to Isaiah Hankel, Cheeky Scientist, help guide you through the different platforms OSA uses and how you can effectively network and get the most out of your meeting experience.

Tuesday, 13 April

Optical Trapping and Manipulation: Careers and Networking Event
Poster Session I

Wednesday, 14 April

Meet the OSA Journals Editors

The OSA Publishing journal Editors welcome your questions, ideas, and concerns. Join this online event to learn more about journal acceptance criteria, responding to review requests, addressing reviewer feedback, and other topics of interest. All are welcome!

Poster Session II

Thursday, 15 April

Photobiomodulation: An emerging biophotonics of clinical transitions and advanced therapeutic devices

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