Optica Biophotonics Congress: Optics in the Life Sciences

Radiation Pressure and Levitated Optomechanics

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1. 8:00 - 8:15

   (UTC - 07:00)
   AW1D.1

   Rydberg Atoms in the Vicinity of an Optical Nanofiber

   Presenter: Etienne Brion, CNRS, Université Toulouse III Paul Sabatier, France

    Presentation Details   Paper

   We investigate how van der Waals interaction between Rydberg atoms is modified in the vicinity of an optical nanofiber with respect to free-space. This work paves the way to promising light-matter interfaces for quantum technologies.
   
   Authors:Erwan Stourm, Université Paris-Saclay / Maxence Lepers, CNRS, Université de Bourgogne Franche-Comté / Jacques Robert, Université Paris-Saclay / Sile Nic Chormaic, Okinawa Institute of Science and Technology Graduate University / Klaus Moelmer, Niels Bohr Institute / Etienne Brion, CNRS, Université Toulouse III Paul Sabatier

2. 8:15 - 8:30

   (UTC - 07:00)
   AW1D.2

   Faster and More Accurate Geometrical-Optics Optical Force Calculation Using Neural Networks

   Presenter: David Bronte Ciriza, CNR-IPCF Messina, Italy

    Presentation Details   Paper

   We use neural networks to improve not only the speed but also the accuracy of the optical force calculations in the geometrical optics approximation. This allows us to study problems that would be computationally impossible otherwise.
   
   Authors:David Bronte Ciriza, CNR-IPCF Messina / Alessandro Magazzù, CNR-IPCF Messina / Agnese Callegari, University of Gothenburg / Gunther Barbosa, Universidade Federal do ABC / Antonio Neves, Universidade Federal do ABC / Maria Iati, CNR-IPCF Messina / Giovanni Volpe, University of Gothenburg / Onofrio Maragò, CNR-IPCF Messina

   Student Paper Finalist Virtual

3. 8:30 - 8:45

   (UTC - 07:00)
   AW1D.3

   Effect of Optical Nonlinearity in Trapping Dynamics of Polystyrene and Silica Micro-Particles Under Femtosecond Pulsed Excitation: a Comparative Study

   Presenter: Arijit De, IISER Mohali, India

    Presentation Details   Paper

   We present a comparative study on optical trapping dynamics for polystyrene and silica micro-particles under both continuous wave and pulsed excitations. The effect of optical nonlinearity under femtosecond pulsed excitation on trapping efficiency is investigated.
   
   Authors:Sumit Yadav, IISER Mohali / Abdul Alim, IISER Mohali / Arijit De, IISER Mohali

   Virtual

4. 8:45 - 9:15

   (UTC - 07:00)
   AW1D.4

   Non-Conservative Levitational Optomechanics: From Stochastic Bifurcations to Synchronization

   Presenter: Stephen Simpson, Institute of Scientific Instruments ASCR, Czechia

    Presentation Details   Paper

   We describe the general stochastic motion of linearly non-conservative optomechanical systems, levitated in vacuum. Theoretical results are illustrated with experiments revealing rich dynamical behaviour, including stochastic bifurcations and synchronization. Applications to sensing are discussed.
   
   Authors:Stephen Simpson, Institute of Scientific Instruments ASCR

   Invited In Person

5. 9:15 - 9:45

   (UTC - 07:00)
   AW1D.5

   Applications of Radiation Pressure Force on a Thin Diffractive Film

   Presenter: Grover Swartzlander, Rochester Institute of Technology, United States

    Presentation Details   Paper

   The radiation pressure force on a diffractive film is described from analytical and experimental points of view, and potential applications are discussed for outer space and in transparent liquids.
   
   Authors:Grover Swartzlander, Rochester Institute of Technology

   Invited

Alignment of Functional Imaging to Circuit Structure and Behavior

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1. 8:00 - 8:15

   (UTC - 07:00)
   BW1B.2

   Optimization of Confocal Bessel Beam Light-Sheet for Axonal Connectomics

   Presenter: Kevin Takasaki, Allen Institute for Brain Science, United States

    Presentation Details   Paper

   Axonal connectomics entails tracing of dense axons over large brain volumes. Here, we describe a light-sheet imaging approach using scanned Bessel beam illumination and confocal slit detection and the results of simulation-guided optimization. We evaluate the performance of our approach for axon resolution and segmentation
   
   Authors:Kevin Takasaki, Allen Institute for Brain Science / Olga Gliko, Allen Institute for Brain Science / Russel Torres, Allen Institute for Brain Science / Pooja Balaram, Allen Institute for Brain Science / Ayana Hellevik, Allen Institute for Brain Science / Emily Turschak, Allen Institute for Brain Science / Bryan MacLennan, Allen Institute for Brain Science / Sam Kinn, Allen Institute for Brain Science / Eric Perlman, Allen Institute for Brain Science / Clay Reid, Allen Institute for Brain Science

   In Person

2. 8:15 - 8:30

   (UTC - 07:00)
   BW1B.3

   Reconstruction of Nerve Fiber Orientations in Cell-Body Stained Histological Brain Sections Using Computational Scattered Light Imaging

   Presenter: Franca auf der Heiden, Forschungszentrum Jülich GmbH, Germany

    Presentation Details   Paper

   We present a method for direct imaging of nerve fiber orientations in cell-body stained histological brain sections, which was not yet possible for paraffin-treated tissue.
   
   Authors:Franca auf der Heiden, Forschungszentrum Jülich GmbH / Marios Georgiadis, Stanford School of Medicine / Michael Zeineh, Stanford School of Medicine / Katrin Amunts, Forschungszentrum Jülich GmbH / Markus Axer, Forschungszentrum Jülich GmbH / Miriam Menzel, Forschungszentrum Jülich GmbH

   In Person

3. 8:30 - 9:00

   (UTC - 07:00)
   BW1B.4

   Leveraging Synthetic Mouse Body Models for Better Alignment of Behavioral Data With Brain Activity

   Presenter: Timothy Murphy, University of British Columbia, Canada

    Presentation Details

   We developed a system to facilitate ethological feature tracking using an animated synthetic mouse for training data for pose-estimation. We aim to automate this process and use this tool to investigate brain activity-behavior connections.
   
   Authors:Timothy Murphy, University of British Columbia

   Invited In Person

4. 9:00 - 9:15

   (UTC - 07:00)
   BW1B.5

   Birefringence Microscope Design and Sample Preparation Exigencies for High-Resolution Imaging of Myelin Degradation

   Presenter: Alexander Gray, Boston University, United States

    Presentation Details   Paper

   Structural imaging of myelin is of utmost importance for characterization of pathological changes to the brain in neurological disorders. Birefringence microscopy is used for rapid visualization of structural changes to myelin at sub-micron (<300nm) resolution.
   
   Authors:Alexander Gray, Boston University / Nathan Blanke, Boston University / Rhiannon Robinson, Boston University School of Medicine / Doug Rosene, Boston University School of Medicine / Irving Bigio, Boston University

   In Person

5. 9:15 - 9:30

   (UTC - 07:00)
   BW1B.6

   Enhanced Determination of Nerve Fiber Orientations by Introducing Iterative Thresholds in Computational Scattered Light Imaging

   Presenter: Miriam Menzel, Forschungszentrum Jülich, Germany

    Presentation Details   Paper

   We improve the determination of nerve fiber orientations in brain tissue sections that have been measured with Computational Scattered Light Imaging by close examination of low intensity signals with iterative thresholding.
   
   Authors:Jan Reuter, Forschungszentrum Jülich / David Gräßel, Forschungszentrum Jülich / Markus Axer, Forschungszentrum Jülich / Katrin Amunts, Forschungszentrum Jülich / Miriam Menzel, Forschungszentrum Jülich

6. 9:30 - 10:00

   (UTC - 07:00)
   BW1B.1

   High Throughput Electron Microscopy-Based Connectomics

   Presenter: Kevin Briggman, MPI Neurobiologie des Verhaltens -CAESAR, Germany

    Presentation Details

   Abstract not available.
   
   Authors:Kevin Briggman, MPI Neurobiologie des Verhaltens -CAESAR

   Invited In Person

Endoscopy and Fiber Optics Sensing

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1. 8:00 - 8:30

   (UTC - 07:00)
   DW1A.1

   Guide Mapping and Endoscopy Cap Approaches for Well-Confined Mucosal Laser Coagulation

   Presenter: Serhat Tozburun, Izmir Biomedicine and Genome Center, Turkey

    Presentation Details   Paper

   Endoscopic management of superficial abnormal tissue in the esophagus remains a valid challenge. With the developments in endoscopic probes and optimization parameters, laser-induced thermal therapy may once again come to the fore as the closest approach.
   
   Authors:Serhat Tozburun, Izmir Biomedicine and Genome Center

   Invited Virtual

2. 8:30 - 9:00

   (UTC - 07:00)
   DW1A.2

   Endoscopic OCT-AFI – in Vivo Imaging of the Lungs, Oral Cavity, and Gynecologic Tract

   Presenter: Pierre Lane, BC Cancer Research Center, Canada

    Presentation Details

   Dual-clad fiber has enabled the simultaneous acquisition of endoscopic optical coherence tomography (OCT) and autofluorescence imaging (AFI). In this talk I present images acquired in vivo from the lung, oral cavity, and gynecologic tract.
   
   Authors:Pierre Lane, BC Cancer Research Center

   Invited In Person

3. 9:00 - 9:15

   (UTC - 07:00)
   DW1A.3

   The Design of Optical Coherence Tomography Probe in Dermatology

   Presenter: Nhan Le, University of Washington, United States

    Presentation Details   Paper

   Abstract: We summarized our recent developments in optical coherence tomography and its utility in clinical settings as a diagnostic and monitoring tool, and in cosmetics as an assessment tool.
   © 2022 The Author(s)
   
   Authors:Nhan Le, University of Washington / Jie Lu, University of Washington / Zhiying Xing, University of Washington / Yaping Shi, University of Washington / Athira B S, University of Washington / Ruikang Wang, University of Washington

   In Person

4. 9:15 - 9:30

   (UTC - 07:00)
   DW1A.4

   Multipath Artifacts in Single-Fiber Endoscopic Multimodal Optical Coherence Tomography Provide Angular Contrast

   Presenter: Adrian Tanskanen, BC Cancer Research Institute, Canada

    Presentation Details   Paper

   A novel method of imaging which exploits multipath image artifact in multimodal OCT is proposed. Experiments explore using the contrast between en face projections of the OCT image and multipath artifact to measure backscattering angle.
   
   Authors:Adrian Tanskanen, BC Cancer Research Institute / Jeanie Malone, BC Cancer Research Institute / Calum MacAulay, BC Cancer Research Institute / Pierre Lane, BC Cancer Research Institute

   In Person

5. 9:30 - 9:45

   (UTC - 07:00)
   DW1A.5

   Broadband Diffuse Reflectance Spectroscopy System With a Self-Calibrating Fiber-Optic Probe

   Presenter: Ilya Turchin, Institute of Applied Physics, Russian Federation

    Presentation Details   Paper

   A VIS-NIR experimental setup for diffuse reflectance spectroscopy with a fiber-optic probe employing a self-calibrating approach has been created and demonstrated high robustness to various instrumental perturbations in source and detector channels.
   
   Authors:Valeriya Perekatova, Institute of Applied Physics / Alexey Kostyuk, Institute of Applied Physics / Mikhail Kirillin, Institute of Applied Physics / Ekaterina Sergeeva, Institute of Applied Physics / Daria Kurakina, Institute of Applied Physics / Olga Shemagina, Institute of Applied Physics / Anna Orlova, Institute of Applied Physics / Ilya Turchin, Institute of Applied Physics

   Virtual

6. 9:45 - 10:00

   (UTC - 07:00)
   DW1A.6

   All-Fiber Photometry Based on a Novel, low-Autofluorescence Wideband Multimode Circulator

   Presenter: Andrey Formozov, Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf, Germany

    Presentation Details   Paper

   Here, we present a wideband multimode circulator optimized for high transfer and transmittance of light, and low autofluoresence. It is suitable for fiber photometry and optogenetics, allowing the inference of brain activity in awake mouse.
   
   Authors:Andrey Formozov, Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf / Joseph Lamarre, Castor Optics, Inc. / Alexander Dieter, Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf / Lucas Majeau, Castor Optics, Inc. / J. Simon Wiegert, Center for Molecular Neurobiology, University Medical Center Hamburg-Eppendorf / Kathy Beaudette, Castor Optics, Inc.

   Virtual

Photoacoustics and Polarization Techniques

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1. 8:00 - 8:15

   (UTC - 07:00)
   NW1C.1

   Dual-Modal Simultaneous Photoacoustic and Harmonic Ultrasound Microscopy

   Presenter: Jingyi Zhu, CITY UNIVERSITY OF HONG KONG, Hong Kong

    Presentation Details   Paper

   We present dual-band photoacoustic and ultrasound microscopy that can maintain high resolution and improve the imaging penetration. The harmonic US imaging on the mouse eye reveals finer lens and iris boundary structures, which offers a high-resolution anatomical reference for co-registered PA imaging.
   
   Authors:Jingyi Zhu, CITY UNIVERSITY OF HONG KONG / Jiangbo Chen, CITY UNIVERSITY OF HONG KONG / Mohammadreza Amjadian, Hong Kong University of Science and Technology / Siyi Liang, CITY UNIVERSITY OF HONG KONG / Zheng Qu, CITY UNIVERSITY OF HONG KONG / Yue Wang, CITY UNIVERSITY OF HONG KONG / Yachao Zhang, CITY UNIVERSITY OF HONG KONG / Lidai Wang, CITY UNIVERSITY OF HONG KONG

2. 8:15 - 8:30

   (UTC - 07:00)
   NW1C.2

   Real-Time Polarimetric Microscopy of Biological Tissue

   Presenter: Ariel Fernández, Universidad de la República, Uruguay

    Presentation Details   Paper

   We present recent advances in Division of Focal Plane polarization sensing for applications to microscopy. By means of illumination engineering and polarization coding, Stokes parameters as well as Mueller matrix of biological samples can be retrieved in a snapshot.
   
   Authors:Ariel Fernández, Universidad de la República / Roman Demczylo, Universidad de la República

   Virtual

3. 8:30 - 8:45

   (UTC - 07:00)
   NW1C.3

   Cross-Polarized Microscopy of Pigmented Cells in Vivo

   Presenter: Rafael Romero, University of Arizona, United States

    Presentation Details   Paper

   We have developed a portable cross-polarized microscope for imaging human skin in vivo at high resolution. Preliminary results showed that high-NA cross-polarized microscopy could visualize individual pigmented cells for the human skin in vivo.
   
   Authors:Rafael Romero, University of Arizona / Jingwei Zhao, University of Arizona / Clara Curiel, University of Arizona / Delaney Stratton, University of Arizona / Dongkyun Kang, University of Arizona

   Virtual

4. 8:45 - 9:00

   (UTC - 07:00)
   NW1C.4

   Four Channel Based Stokes-Mueller Polarimetry for Tissue Characterization Integrated With Machine Learning

   Presenter: Nirmal Mazumder, Manipal Academy of Higher Education, India

    Presentation Details   Paper

   We propose a transmission-based Stokes-Mueller microscope for quantitative analysis of the microstructural properties of the tissue specimen. Further, by applying a suitable image processing technique such as machine learning (ML) output images were analysed effectively.
   
   Authors:Spandana K U, Manipal Academy of Higher Education / Sindhoora Kaniyala Melanthota, Manipal Academy of Higher Education / Raghavendra U, Manipal Academy of Higher Education / Sharada Rai, Manipal Academy of Higher Education / K K Mahato, Manipal Academy of Higher Education / Nirmal Mazumder, Manipal Academy of Higher Education

5. 9:00 - 9:30

   (UTC - 07:00)
   NW1C.7

   Photoacoustic Remote Sensing Virtual Histology

   Presenter: Roger Zemp, University of Alberta, Canada

    Presentation Details

   Photoacoustic Remote Sensing (PARS) Microscopy is a fundamentally new form of all-optical microscopy that offers optical absorption contrast in a reflection mode geometry. We highlight recent work demonstrating realistic PARS virtual histology in combination with scattering and fluorescence as well as optical coherence tomography.
   
   Authors:Roger Zemp, University of Alberta

   Invited In Person

6. 9:30 - 9:45

   (UTC - 07:00)
   NW1C.5

   (Withdrawn) Layered Phase-Fourier Polarization Scanning of Object Fields of Blood Films in Differential Diagnosis of Prostate Cancer

   Presenter: Olexander Dubolazov, Chernivtsi National University, Ukraine

    Presentation Details

   Physically substantiated and experimentally determined the effectiveness of the 3D method of direct and with spatial-frequency filtering of layered polarization mapping of polycrystalline films of blood in differentiating malignant conditions of prostate tissue.
   
   Authors:Olexander Dubolazov, Chernivtsi National University / Olexander Ushenko, Chernivtsi National University / Yuriy Ushenko, Chernivtsi National University / Iryna Soltys, Chernivtsi National University / Jun Zheng, Research Institute of Zhejiang University / Lin Xianghua, Research Institute of Zhejiang University / Zhebo Chen, Research Institute of Zhejiang University / Ivan Mikirin, Chernivtsi National University / N Pavlyukovich, Bukovinian State Medical University

7. 9:45 - 10:00

   (UTC - 07:00)
   NW1C.6

   (Withdrawn) 3D Polarization Interference Metrology Polycrystalline Films of Cerebrospinal Fluid in the Diagnosis of the Time of Death

   Presenter: Olexander Dubolazov, Chernivtsi National University, Ukraine

    Presentation Details

   The forensic efficiency of the 3D polarization mapping of liquor layers in determining the antiquity of the onset of death (OOD) is considered. The possibility of precision (15 min) determination of OOD has been demonstrated.
   
   Authors:Olexander Dubolazov, Chernivtsi National University / Olexander Ushenko, Chernivtsi National University / Yuriy Ushenko, Chernivtsi National University / Iryna Soltys, Chernivtsi National University / Jun Zheng, Research Institute of Zhejiang University / Lin Xianghua, Research Institute of Zhejiang University / Zhebo Chen, Research Institute of Zhejiang University / A Salega, Chernivtsi National University / M Garazdyuk, Bukovinian State Medical University

Preclinical Applications of Optical Molecular Imaging and Spectroscopy

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1. 8:00 - 8:30

   (UTC - 07:00)
   OW1E.1

   Surface Enhanced Spatially Offset Raman Spectroscopy (SESORS) for Preclinical Cancer Imaging

   Presenter: Fay Nicolson, Dana-Farber Cancer Institute, United States

    Presentation Details

   We discuss the optimization of spatially offset Raman instrumentation and imaging approaches, and subsequent application of SESORS to preclinical cancer imaging and delineation of tumor margins in mouse models of colorectal cancer and glioblastoma.
   
   Authors:Fay Nicolson, Dana-Farber Cancer Institute / Eunah Lee, Horiba Scientific / Andrew Whitley, Horiba Scientific / Scott Rudder, Optosigma / Moritz Kircher, Dana-Farber Cancer Institute / Kevin Haigis, Dana-Farber Cancer Institute

   Invited In Person

2. 8:30 - 9:00

   (UTC - 07:00)
   OW1E.2

   Imaging and Sensing of Biomolecular Processes and Pathways in Primary Tumors to Identify Long-Term Outcome

   Presenter: Narasimhan Rajaram, University of Arkansas, United States

    Presentation Details

   This talk will discuss recent work from the lab utilizing Raman spectroscopy and two-photon microscopy to measure biomolecular and metabolic profiles of primary human and mouse tumors to evaluate long-term outcome.
   
   Authors:Narasimhan Rajaram, University of Arkansas

   Invited In Person

3. 9:00 - 9:15

   (UTC - 07:00)
   OW1E.3

   Development of a Multi-Scale Platform to Quantify Metabolic Heterogeneity in Vivo

   Presenter: Riley Deutsch, Duke University, United States

    Presentation Details   Paper

   We developed an approach to quantify intratumoral metabolic heterogeneity of in vivo tumor models by leveraging a computationally designed multi-scale microscope and a suite of exogenous fluorescent contrast agents to provide functional and structural information.
   
   Authors:Riley Deutsch, Duke University / Roujia Wang, Duke University / Enakshi Sunassee, Duke University / Megan Madonna, Duke University / Brian Crouch, Duke University / Nirmala Ramanujam, Duke University

   In Person

4. 9:15 - 9:30

   (UTC - 07:00)
   OW1E.4

   In Vivo Metabolic Imaging Reveals Mitochondrial Membrane Potential Reprogramming During Residual Disease Following Chemotherapy

   Presenter: Enakshi Sunassee, Duke University, United States

    Presentation Details   Paper

   We performed intravital fluorescent microscopy in preclinical models of disease regression in Triple Negative Breast Cancer to compare dynamic, long-term in vivo changes across three major metabolic axes in chemotherapy-resistant and chemotherapy-sensitive tumors.
   
   Authors:Enakshi Sunassee, Duke University / Brian Crouch, Duke University / Megan Madonna, Duke University / Gregory Palmer, Duke University / Nirmala Ramanujam, Duke University

   In Person

5. 9:30 - 9:45

   (UTC - 07:00)
   OW1E.5

   Fluorescent Molecular Labeling and in Vivo Detection of Circulating Tumor Cells in Mice

   Presenter: Josh Pace, Northeastern University, United States

    Presentation Details   Paper

   The goal of this research is to develop a method to label, detect, and count circulating tumor cells directly in vivo with an injectable folate-receptor targeted fluorescent molecular probe and near infrared (NIR) light.
   
   Authors:Josh Pace, Northeastern University / Srinivasarao Madduri, Purdue University / Shivakrishna Kallepu, Purdue University / Philip Low, Purdue University / Mark Niedre, Northeastern University

   In Person

6. 9:45 - 10:00

   (UTC - 07:00)
   OW1E.6

   Measuring in Vivo Dissociation Kinetics of Porphysome Nanoparticles Using Diffuse Reflectance and Quantitative Fluorescence Spectroscopy

   Presenter: Theodore Husby, University of Toronto, Canada

    Presentation Details   Paper

   A combination diffuse reflectance and quantitative fluorescence spectroscopy system developed for measuring concentrations of intact and dissociated states of porphysome theranostic nanoparticles is compared to pre-clinical ex vivo PET measurements and in vivo dissociation kinetics.
   
   Authors:Theodore Husby, University of Toronto / Michael Valic, University of Toronto / Yoshihisa Hiraishi, Toronto General Hospital / Nicholas Bernards, Toronto General Hospital / Fumi Yokote, Toronto General Hospital / Juan Chen, University Health Network / Gang Zheng, University Health Network / Kazuhiro Yasufuku, Toronto General Hospital / Brian Wilson, University Health Network / Robert Weersink, University Health Network

   In Person

Exhibit Hall and Coffee Break

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Joint Postdeadline Paper I

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1. 10:30 - 10:45

   (UTC - 07:00)
   JW2A.1

   Measuring Brain Function During Motor Imitation With High-Density Diffuse Optical Tomography (HD-DOT)

   Presenter: Tessa George, Washington University School of Medicine, United States

    Presentation Details   Paper

   We utilized HD-DOT to image fifty adults as they observed and imitated sequences of upper extremity movements. We show HD-DOT is resilient to movement and successfully maps spatially distributed brain function during gross motor movements. © 2022 The Author(s).
   
   Authors:Tessa George, Washington University School of Medicine / Dalin Yang, Washington University School of Medicine / Kelsey King, Roosevelt University / Sophia McMorrow, Washington University School of Medicine / Chloe Sobolewski, Washington University School of Medicine / Bahar Tuncgenc, University of Nottingham / Mary Beth Nebel, Kennedy Krieger Institute / Natasha Marrus, Washington University School of Medicine / Stewart Mostofsky, Kennedy Krieger Institute / Adam Eggebrecht, Washington University School of Medicine

   In Person

2. 10:45 - 11:00

   (UTC - 07:00)
   JW2A.2

   Measurements of Slow Tissue Dynamics With Short-Separation Speckle Contrast Optical Spectroscopy

   Presenter: Xiaojun Cheng, Boston University, United States

    Presentation Details   Paper

   We present a short-separation speckle contrast optical spectroscopy (ss-SCOS) system to enhance the static and slow tissue components with the multi-exposure time based speckle contrast model to extract both the slow tissue and fast blood flow dynamics.
   
   Authors:Bingxue Liu, Boston University / David Boas, Boston University / Xiaojun Cheng, Boston University

   In Person

3. 11:00 - 11:15

   (UTC - 07:00)
   JW2A.3

   Breaking new Ground in Protein Detection: Self-Supervised Machine Learning and ISCAT Enable Label-Free Detection of Single Proteins Below 10 kDa

   Presenter: Mahyar Dahmardeh, Max Planck Institute for the Science of, Germany

    Presentation Details   Paper

   Interferometric scattering (iSCAT) microscopy detects single nanoparticles in a label-free fashion. Utilizing self-supervised machine learning pushes the detection sensitivity of iSCAT to very small proteins and disease markers such as chemokines and cytokines.
   
   Authors:Mahyar Dahmardeh, Max Planck Institute for the Science of / Houman Mirzaalian Dastjerdi, Max Planck Institute for the Science of / Hisham Mazal, Max Planck Institute for the Science of / Harald Köstler, Department of Computer Science, Friedrich-Alexander-Universitat Erlangen-Nurnberg / Vahid Sandoghdar, Max Planck Institute for the Science of

   In Person

4. 11:15 - 11:30

   (UTC - 07:00)
   JW2A.4

   Collinear Ultrasonic and Multiphoton Focusing Reveals New Effects and Image Details

   Presenter: Shy Shoham, New York University, United States

    Presentation Details   Paper

   We report on a novel, strong acousto-optic modulation of two-photon excited fluorescence. This effect enables enhanced two photon imaging in deep brain tissue in vivo.
   
   Authors:Ezra Guralnik, New York University / Behnam Tayebi, Inscopix / Yi Yuan, Institute of Electronic Engineering / Justin Little, NYU Grossman School of Medicine / Michal Balberg, Holon Institute of Technology / Shy Shoham, New York University

Joint Postdeadline Paper II

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1. 10:30 - 10:45

   (UTC - 07:00)
   JW2B.1

   In Vivo Stiffness Mapping of the Human Cornea Using High-Frequency Optical Coherence Elastography

   Presenter: Xu Feng, Massachusetts General Hospital, United States

    Presentation Details   Paper

   Measuring the spatial variation of the cornea has clinical significance for diagnosing corneal ectasia disease and evaluating refractive surgery outcomes. We present spatially-resolved stiffness mapping of the human cornea in vivo using optical coherence elastography.
   
   Authors:Xu Feng, Massachusetts General Hospital / Guo-Yang Li, Massachusetts General Hospital / Antoine Ramier, Massachusetts General Hospital / Seok-Hyun Yun, Massachusetts General Hospital

2. 10:45 - 11:00

   (UTC - 07:00)
   JW2B.2

   Second Harmonic Generation Transmission Matrix Microscopy for Computational Aberration Correction

   Presenter: Cho Ye-chan, Institute for Basic Science, Korea (the Republic of)

    Presentation Details   Paper

   We introduce a method to record the transmission matrix of second-harmonic signals, and computationally correct sample-induced aberration. We demonstrated proposed concept by imaging a target under an aberration layer and visualizing muscle tissue in zebrafish.
   
   Authors:Cho Ye-chan, Institute for Basic Science / Jungho Moon, Institute for Basic Science / Sungsam Kang, Institute for Basic Science / Wonshik Choi, Institute for Basic Science

   In Person

3. 11:00 - 11:15

   (UTC - 07:00)
   JW2B.3

   Ultralow Power Refractive Index Tapered Optical Fiber Glucose Sensor

   Presenter: Meimei Lai, ODU Center for Bioelectronics, United States

    Presentation Details   Paper

   Tapered optical fiber (TOF) was successfully fabricated for refractive index glucose sensing. AuNPs decorated TOF introduced localized surface plasmon resonance. Optical transmission with an ultralow-power beam showed AuNPs enhanced the sensitivity by 4 times (2032%/RIU).
   
   Authors:Meimei Lai, ODU Center for Bioelectronics / Erem Ujah, ODU Center for Bioelectronics / Gymama Slaughter, ODU Center for Bioelectronics

4. 11:15 - 11:30

   (UTC - 07:00)
   JW2B.4

   Unveiling the 3D Cytoarchitecture of the Human Cerebellum With Multimodal Tomography Using Near-Field Speckles at the Synchrotron

   Presenter: Sara Savatovic, University of Trieste, Italy

    Presentation Details   Paper

   Speckle-based imaging provides complementary information through multiple imaging channels for three-dimensional visualisation of cerebellar cortex tissues. We describe a preliminary investigation of multi-resolution tomography, down to single cells, of the human cerebellum.
   
   Authors:Sara Savatovic, University of Trieste / Marie-Christine Zdora, Paul Scherrer Institut / Fabio De Marco, University of Trieste / Christos Bikis, Psychiatric hospital in Winterthur / Margie Olbinado, Paul Scherrer Institut / Alexander Rack, ESRF – The European Synchrotron / Bert Müller, University of Basel / Pierre Thibault, University of Trieste / Irene Zanette, Elettra-Sincrotrone Trieste

   In Person

5. 11:30 - 11:45

   (UTC - 07:00)
   JW2B.5

   Intrinsic Stochastic Fluorescence Spectra and Photophysical Parameters of Nucleic Acids Under Visible Light Illumination

   Presenter: Ruyi Gong, Northwestern University, United States

    Presentation Details   Paper

   The spectra and photophysical parameters of DNA molecules under visible light illumination of different wavelengths are measured for the first time, which may lead to a new label-free super-resolution imaging technique.
   
   Authors:Ruyi Gong, Northwestern University / Luay Almassalha, Northwestern University / Vadim Backman, Northwestern University

   Virtual

Clinical Translation of Optical Molecular Imaging and Image Guide Surgery

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1. 13:45 - 14:15

   (UTC - 07:00)
   OW3E.2

   Fluorescence Guided Surgery

   Presenter: Kimberley Samkoe, Dartmouth College, United States

    Presentation Details

   Abstract not available.
   
   Authors:Kimberley Samkoe, Dartmouth College

   Invited In Person

2. 14:15 - 14:30

   (UTC - 07:00)
   OW3E.3

   Margin Status Assessment Using a Ratio-Metric Angular Domain Fluorescent Imaging Approach in Patients With Head and Neck Squamous Cell Carcinoma

   Presenter: Cody Rounds, Illinois Institute of Technology, United States

    Presentation Details   Paper

   Many head and neck squamous cell carcinoma surgical patients are left with residual tumor post excision surgery (inadequate margins). We present an imaging strategy capable of rapid margin status assessment to reduce overall surgical burden.
   
   Authors:Cody Rounds, Illinois Institute of Technology / Jaron de Wit, University Medical Center Groningen / Jasper Vonk, University Medical Center Groningen / Floris Voskuil, University Medical Center Groningen / Max Witjes, University Medical Center Groningen / Kenneth Tichauer, Illinois Institute of Technology

   In Person

3. 14:30 - 15:00

   (UTC - 07:00)
   OW3E.4

   Clinical Fluorescein Imaging to Detect Early Signs of Diabetic Retinopathy

   Presenter: Kenneth Tichauer, Illinois Institute of Technology, United States

    Presentation Details

   80% of individuals with diabetes experience diabetic retinopathy within their lifetimes. This work demonstrates that subtle early changes in retinal vascular permeability can be detected with fluorescein videoangiography as a potential biomarker of diabetic retinopathy.
   
   Authors:Kenneth Tichauer, Illinois Institute of Technology

   Invited In Person

4. 15:00 - 15:30

   (UTC - 07:00)
   OW3E.5

   Real-Time Surgical Assessment Using an Ambient-Light Compatible Wide-Field Fluorescence-Guided Surgery Platform

   Presenter: Christie Lin, OnLume Surgical, United States

    Presentation Details

   I will describe recent clinical research using our wide-field fluorescence-guided surgery imaging device, ranging from microsurgical reconstruction to wound debridement, and identify future surgical applications.
   
   Authors:Christie Lin, OnLume Surgical

   Invited In Person

5. 15:30 - 16:00

   (UTC - 07:00)
   OW3E.1

   Development and Clinical Application of SGM-101, a Fluorescent Anti-CEA Antibody, for Real-Time Detection of Tumors During Fluorescence-Guided Surgery

   Presenter: Françoise Cailler, SurgiMab, France

    Presentation Details

   Discriminating between malignant and benign tissue remains the biggest challenge during oncology surgery. We will describe the main steps of the clinical translation of SGM-101, from an exciting concept to a molecule used by clinicians.
   
   Authors:Françoise Cailler, SurgiMab / Bérénice Framery, SurgiMab / Mats Warmerdam, LUMC / Alexander Vahrmeijer, LUMC

   Invited In Person

Environmental Applications

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1. 14:00 - 14:30

   (UTC - 07:00)
   AW3D.1

   Optical Trapping of Aerosols

   Presenter: Grégory David, ETH Zurich, Switzerland

    Presentation Details

   Novel experiments are developed to characterize in details optically-trapped aerosol particles. The time evolution of the measured particle properties are then used to study diverse processes including photochemistry, condensation of water, hygroscopicity and phase transitions.
   
   Authors:Grégory David, ETH Zurich

   Invited In Person

2. 14:30 - 14:45

   (UTC - 07:00)
   AW3D.2

   Advanced Algorithms for Bacterial Analysis in Body Fluids Using Raman Tweezers

   Presenter: Ondrej Vaculik, Institute of Scientific Instruments of the Czech Academy of Sciences, Czechia

    Presentation Details   Paper

   Analysis of bacteria in body fluids using Raman tweezers generates a mixture of signals from the bacteria and the medium. Advanced spectral unmixing algorithms (PCA, k-means, FIPPI, N-FINDR) can be used to separate each signal.
   
   Authors:Ondrej Vaculik, Institute of Scientific Instruments of the Czech Academy of Sciences / Silvie Bernatova, Institute of Scientific Instruments of the Czech Academy of Sciences / Mojmir Sery, Institute of Scientific Instruments of the Czech Academy of Sciences / Martin Siler, Institute of Scientific Instruments of the Czech Academy of Sciences / Ota Samek, Institute of Scientific Instruments of the Czech Academy of Sciences / Katarina Rebrosova, Faculty of Medicine of Masaryk University and St. Anne's, University Hospital / Filip Ruzicka, Faculty of Medicine of Masaryk University and St. Anne's, University Hospital / Pavel Zemanek, Institute of Scientific Instruments of the Czech Academy of Sciences

   In Person

3. 14:45 - 15:00

   (UTC - 07:00)
   AW3D.3

   Optical Tweezers With Integrated Multiplane Microscopy

   Presenter: Lynn Paterson, Heriot-Watt University, United Kingdom

    Presentation Details   Paper

   Optical tweezer have been combined with multiplane microscopy to measure particle trajectories in three dimensions and to calculate the viscosity of the suspending media in three dimensions at micrometer length scales. The method is applicable to biological samples.
   
   Authors:Lynn Paterson, Heriot-Watt University / Andrew Matheson, Heriot-Watt University / Amanda Wright, University of Nottingham / Tania Mendonca, University of Nottingham / Manlio Tassieri, University of Glasgow / Paul Dalgarno, Heriot-Watt University

   In Person

4. 15:00 - 15:30

   (UTC - 07:00)
   AW3D.4

   Optical and Raman Tweezers for the Manipulation and Characterization of Cosmic Dust and Sea Microplastics

   Presenter: Onofrio Maragò, CNR-IPCF, Italy

    Presentation Details   Paper

   We use optical and Raman Tweezers to manipulate micro and nano-plastics and individual cosmic dust particles. We identify their compositions and shapes studying their response to optical forces, enabling a better understanding of the plastics fragmentation processes in sea environment and the effects of light on single grains of cosmic dust.
   
   Authors:Alessandro Magazzù, CNR-IPCF / Raymond Gillibert, CNR-IPCF / David Bronte Ciriza, CNR-IPCF / Anna Musolino, CEREGE Aix-Marseille Université / Maria Grazia Donato, CNR-IPCF / Antonino Foti, CNR-IPCF / Lamy de la Chapelle, Le Mans Universitè / Fabienne Lagarde, Le Mans Universitè / Alessandra Rotundi, Università degli studi di Napoli Parthenope / Luigi Folco, Università di Pisa / Onofrio Maragò, CNR-IPCF / Pietro Giuseppe Gucciardi, CNR-IPCF

   Invited

High Speed and Adaptive Microscopy

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1. 14:00 - 14:15

   (UTC - 07:00)
   BW3B.2

   High-Speed Two-Photon Microscopy With Adaptive Sampling

   Presenter: Weijian Yang, University of California, Davis, United States

    Presentation Details   Paper

   We demonstrate a high-speed two-photon fluorescence microscope using line illumination with an adaptive sampling scheme. The illumination pattern is modulated by a digital micro-mirror device so only the regions of interest are illuminated and sampled.
   
   Authors:Yunyang Li, University of California, Davis / Junjie Hu, University of California, Davis / Shu Guo, University of California, Davis / Weijian Yang, University of California, Davis

   In Person

2. 14:15 - 14:30

   (UTC - 07:00)
   BW3B.3

   DEEP²: Deep Learning Powered De-Scattering With Excitation Patterning (DEEP)

   Presenter: Dushan Wadduwage, Harvard University, United States

    Presentation Details   Paper

   We present DEEP², a computational multiphoton microscope to image through scattering tissue. In DEEP², temporally focused structured light excites deep tissue in wide-field, and deep learning reconstructs clean images from scattered measurements.
   
   Authors:Dushan Wadduwage, Harvard University

   In Person

3. 14:30 - 15:00

   (UTC - 07:00)
   BW3B.4

   Imaging the Mammalian Brain Across Space and Time With Multi-Foci Multi-Photon Microscopy

   Presenter: Jerry Chen, Boston University, United States

    Presentation Details

   The need to image the brain at cellular resolution across large areas of the brain and at higher temporal resolution pushes the boundaries of optical imaging. I will discuss solutions that overcome these fundamental limits.
   
   Authors:Jerry Chen, Boston University

   Invited In Person

4. 15:00 - 15:15

   (UTC - 07:00)
   BW3B.5

   Differentiable Adaptive Optics and Light Shaping in Two-Photon Microscopy

   Presenter: Ivan Vishniakou, Max Planck Institute for Neurobiology, Germany

    Presentation Details   Paper

   We develop a differentiable optics approach for modeling light propagation through a high N.A. microscope objective based on efficient computational optimization of the Debye-Wolf diffraction integral. We apply the technique for wavefront sensing for adaptive optics without guide stars and light shaping in a two-photon microscope.
   
   Authors:Ivan Vishniakou, Max Planck Institute for Neurobiology / Johannes Seelig, Max Planck Institute for Neurobiology

   Virtual

5. 15:15 - 15:30

   (UTC - 07:00)
   BW3B.6

   Imaging of Dendrites and Sparse Interneuronal Networks With 3D Random Access Microscopy

   Presenter: Balázs Chiovini, Pázmány Péter University, Hungary

    Presentation Details   Paper

   Acousto-optical microscopy is a powerful tool to study spare networks and dendritic arborization from the cortex of behaving mice. This novel approach was used for imaging sparse interneuron populations in a combination with auditory discrimination and detection tasks. Our results shed light of novel network mechanisms in multiple brain regions.
   
   Authors:Balázs Chiovini, Pázmány Péter University / Zoltán Szadai, Inst Exp Medicine, Hungarian Acad Sci / Linda Judák, Pázmány Péter University / Balázs Chiovini, Pázmány Péter University / Gábor Juhász, The Faculty of Information Technology, Pázmány Péter University / Katalin Ócsai, The Faculty of Information Technology, Pázmány Péter University / Dénes Pálfi, The Faculty of Information Technology, Pázmány Péter University / Zsolt Mezriczky, The Faculty of Information Technology, Pázmány Péter University / Gergely Szalay, Pázmány Péter University / Gergely Katona, The Faculty of Information Technology, Pázmány Péter University / Anna Mihály, The Faculty of Information Technology, Pázmány Péter University / Áron Szepesi, Pázmány Péter University / Zoltán Mucsi, BrainVisionCenter / Botond Roska, BrainVisionCenter / Hyun-Jae Pi, Cold Spring Harbor Laboratory / Quentin Chevy, Cold Spring Harbor Laboratory / Florin Albeanu, Cold Spring Harbor Laboratory / Adam Kepecs, Cold Spring Harbor Laboratory

   In Person

6. 15:30 - 16:00

   (UTC - 07:00)
   BW3B.1

   High Speed Volumetric Imaging

   Presenter: Elizabeth Hillman, Columbia University, United States

    Presentation Details

   Oblique light-sheet methods can enable ultra-fast imaging of 3D volumes. A simple single-objective geometry and low phototoxicity permits imaging of 3D structure and function in diverse specimens. Technological advances and new applications will be described.
   
   Authors:Elizabeth Hillman, Columbia University

   Invited In Person

Polarization-Based Imaging and Modelling Techniques

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1. 14:00 - 14:15

   (UTC - 07:00)
   DW3A.1

   Deep Brain Polarization Sensitive Optical Coherence Tomography: Depth Resolved Retardance and Optic Axis Measurement

   Presenter: Shadi Masoumi, cervo Brain research center, Canada

    Presentation Details   Paper

   We present improved reconstruction of depth-resolved optic axis orientation in
   catheter-based polarization-sensitive optical coherence tomography, by using the intrinsic
   retardance of the catheter-sheath as reference, and image white matter fiber bundles in sheep
   brain.
   
   Authors:Shadi Masoumi, cervo Brain research center / Georgia Jones, Harvard Medical School, Boston, MA 02115, USA / Jaeyul lee, Harvard Medical School, Boston, MA 02115, USA / Mireille Quémener, cervo Brain research center / lida Hariri, Harvard Medical School, Boston, MA 02115, USA / martin parent, cervo Brain research center / Daniel Côté, cervo Brain research center / Martin Villiger, Harvard Medical School, Boston, MA 02115, USA

   In Person

2. 14:15 - 14:30

   (UTC - 07:00)
   DW3A.3

   Mueller Matrix Measurement Using Faraday Rotator-Based Fiber Polarization-Sensitive Optical Coherence Tomography

   Presenter: Athira B S, University of Washington, United States

    Presentation Details   Paper

   We propose a scheme for polarization-sensitive measurements with fiber-based optical coherence tomography based on the nonreciprocal behavior of the Faraday rotator to measure the 3 X 3 Mueller matrix of the sample.
   
   Authors:Athira B S, University of Washington / Nirmalya Ghosh, IISER Kolkata / Nhan Minh Le, University of Washington / Ruikang Wang, University of Washington

   Virtual

3. 14:30 - 14:45

   (UTC - 07:00)
   DW3A.5

   Investigating Skin Aging Using Jones Matrix Polarization-Sensitive Optical Coherence Tomography

   Presenter: Sina Maloufi, UBC, Canada

    Presentation Details   Paper

   JM-OCT was utilized to present comparison on the effects of intrinsic aging on features quantified by JM-OCT, such as epidermal thickness, local depolarization, and collagen disruption.
   
   Authors:Sina Maloufi, UBC / Xin Zhou, UBC / Mitra Mirsalehi, UBC / Daniel Louie, UBC / Tim Lee, UBC / Shuo Tang, UBC

   In Person

4. 14:45 - 15:00

   (UTC - 07:00)
   DW3A.6

   Towards Point-of-Care Diagnostics and Monitoring of Hypertensive Episodes (a Monte Carlo Approach)

   Presenter: Akhil Kallepalli, University of Glasgow, United Kingdom

    Presentation Details   Paper

   We present an assessment of photon transport through healthy and hypertensive tissue models using Monte Carlo methods. The tissue models (including skin layers and blood vessels) are assessed under red wavelength illumination in TracePro.
   
   Authors:Mark Main, University of Glasgow / Akhil Kallepalli, University of Glasgow

   In Person

5. 15:00 - 15:15

   (UTC - 07:00)
   DM3A.2

   Digital Calibration Method Enables Depth-Resolved all-Fiber Polarization Sensitive Optical Coherence Tomography With an Arbitrary Input Polarization State

   Presenter: Ruikang Wang, University of Washington, United States

    Presentation Details   Paper

   We report a fully integrated all fiber-based polarization sensitive optical coherence tomography (PSOCT) system, allowing depth-resolved and polarization mode dispersion free PSOCT images with single arbitrary input polarization state.
   
   Authors:Peijun Tang, University of Washington / Ruikang Wang, University of Washington

   In Person

6. 15:15 - 15:30

   (UTC - 07:00)
   DW3A.2

   (Withdrawn) Polarization Singular Investigation of Mueller-Matrix Images in the Diagnosis of Local Changes in the Bio Polycrystalline Component of Phase-Inhomogeneous Layers

   Presenter: Olexander Dubolazov, Chernivtsi National University, Ukraine

    Presentation Details

   The paper presents the possibilities of the polarization-singular approaches to increase the efficiency of differential Mueller-matrix polarimetry diagnosis of histological sections of biopsy of adenoma and adenocarcinoma of the prostate with excellent balanced accuracy.
   
   Authors:Olexander Dubolazov, Chernivtsi National University / Olexander Ushenko, Chernivtsi National University / Yuriy Ushenko, Chernivtsi National University / Mykhailo Gorsky, Chernivtsi National University / Jun Zheng, Research Institute of Zhejiang University / Zhebo Chen, Research Institute of Zhejiang University / Lin Xianghua, Research Institute of Zhejiang University / V. Polyvkan, Bukovinian State Medical University / O. Bakun, Bukovinian State Medical University

7. 15:30 - 15:45

   (UTC - 07:00)
   DW3A.4

   (Withdrawn) 3D Digital Holographic Jones Matrix Microscopy of Polycrystalline Architectonics of Dehydrated Biological Fluid Films

   Presenter: Olexander Dubolazov, Chernivtsi National University, Ukraine

    Presentation Details

   Physically substantiated and experimentally determined the effectiveness of the method of 3D layered Jones-matrix mapping of preparations of polycrystalline films of blood plasma and saliva in the diagnosis of malignant conditions of prostate tissue.
   
   Authors:Olexander Dubolazov, Chernivtsi National University / Olexander Ushenko, Chernivtsi National University / Yuriy Ushenko, Chernivtsi National University / Iryna Soltys, Chernivtsi National University / Jun Zheng, Research Institute of Zhejiang University / Zhebo Chen, Research Institute of Zhejiang University / Lin Xianghua, Research Institute of Zhejiang University / I Gordey, Bukovinian State Medical University / Olexander Pavlyukovich, Bukovinian State Medical University / O Litvinenko, Bukovinian State Medical University

8. 15:45 - 15:45

   (UTC - 07:00)
   DW3A.7

   (Withdrawn) Modelling and in-Vitro Study of Ultrashort-Pulsed Laser Irradiation on Human Enamel for Tooth Restoration: a Hybrid Technique

   Presenter: Sarathkumar Loganathan, University of Leeds, United Kingdom

    Presentation Details

   An ultrashort pulsed laser is an emerging non-invasive tool for performing high-precision surgery in restorative dentistry. The present study demonstrated the employability of ultrashort-pulsed laser as a hybrid tool for both tooth preparation and filling damaged tooth enamels.
   
   Authors:Sarathkumar Loganathan, University of Leeds / Eric Barimah, University of Leeds / Neelam Iqbal, University of Leeds / Simon Strafford, University of Leeds / Brian Nattress, University of Leeds / Sue Pavitt, University of Leeds / Anna Nielson, University of Leeds / Animesh Jha, University of Leeds

   Virtual

3D Tomography and Scattering Contrast

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1. 14:00 - 14:30

   (UTC - 07:00)
   NW3C.1

   3D Tomography of Scattering Tissue

   Presenter: Shwetadwip Chowdhury, University of Texas at Austin, United States

    Presentation Details

   I present our recent developments towards 3D label-free imaging of scattering samples. I will discuss the computational frameworks that underpin these developments. Lastly, I will discuss recent results and future directions when applying this methodology in practice.
   
   Authors:Shwetadwip Chowdhury, University of Texas at Austin

   Invited In Person

2. 14:30 - 14:45

   (UTC - 07:00)
   NW3C.3

   Laser Speckle Imaging With Tilted Plane Illumination

   Presenter: Nanguang Chen, National University of Singapore, Singapore

    Presentation Details   Paper

   
   We have developed a laser speckle imaging method based on selected plane illumination. Optical sectioning is achieved for 3D quantitative flow imaging.
   
   Authors:Kai Long, National University of Singapore / Shuhao Shen, National University of Singapore / E Du, National University of Singapore / Nanguang Chen, National University of Singapore

   In Person

3. 14:45 - 15:00

   (UTC - 07:00)
   NW3C.4

   Using Angular Light Scattering to Measure Protein Aggregation

   Presenter: Cassidy Vik, Rollins College, United States

    Presentation Details   Paper

   Preliminary investigations were conducted to determine whether light scattering from proteins could be measured and fit to Mie theory. Distinct differences were observed in the scattering patterns from various aggregation sizes.
   
   Authors:Cassidy Vik, Rollins College / Lani Espinel, Rollins College / Ashley Cannaday, Rollins College

   In Person

4. 15:00 - 15:30

   (UTC - 07:00)
   NW3C.2

   Solving Higher Order Inverse Scattering Problem for in Vivo Through-Skull Imaging

   Presenter: Wonshik Choi, Korea University, Korea (the Republic of)

    Presentation Details

   We developed a method to keep track of multiple light scattering events from the experimentally measured reflection matrix and demonstrated in vivo through-skull imaging of a mature mouse brain.
   
   Authors:Wonshik Choi, Korea University

   Invited In Person

5. 15:30 - 16:00

   (UTC - 07:00)
   NW3C.5

   Scan-Free Stimulated Raman Scattering Tomography for Volumetric Deeper Tissue Imaging

   Presenter: Zhiwei Huang, National University of Singapore, Singapore

    Presentation Details   Paper

   We report on the development of a unique z-scan-free stimulated Raman scattering tomography technique for volumetric deeper tissue imaging.
   
   Authors:Zhiwei Huang, National University of Singapore

   Invited In Person

Exhibit Hall and Coffee Break

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Non-Linear Optics and Upconversion

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1. 16:30 - 17:00

   (UTC - 07:00)
   AW4D.1

   Multiparametric Remote Optical Sensing by a Single Trap Upconverting Microparticle at the Microscale

   Presenter: Patricia Haro Gonzalez, Universidad Autonoma de Madrid, Spain

    Presentation Details   Paper

   Control over particles is key for bio-applications. Rotation dynamics analysis allows the medium characterization. An optically trapped and rotated microparticle is presented as sensor to characterize the properties of a liquid medium at the microscale
   
   Authors:Elisa Ortiz Rivero, Universidad Autonoma de Madrid / Daniel Jaque, Universidad Autonoma de Madrid / Patricia Haro Gonzalez, Universidad Autonoma de Madrid

   Invited In Person

2. 17:00 - 17:15

   (UTC - 07:00)
   AW4D.2

   Accelerating Self-Assembly of Colloidal Particles at Air-Water Interface by Laser-Induced Heating of Upconverting Particle

   Presenter: Muruga Lokesh, Indian Institute of Technology Madras, India

    Presentation Details   Paper

   Optically induced heating of a trapped upconverting particle at the air-water interface of a sessile water droplet creates fluid flows which accelerate the self-assembly of suspended colloidal particles to form a lattice-like structure
   
   Authors:Muruga Lokesh, Indian Institute of Technology Madras / Gokul Nalupurackal, Indian Institute of Technology Madras / Pallab Mahapatra, Indian Institute of Technology Madras / Basudev Roy, Indian Institute of Technology Madras / Imdad Chowdhury, Indian Institute of Technology Madras

3. 17:15 - 17:30

   (UTC - 07:00)
   AW4D.3

   Simultaneous Optical Trapping and Magnetic Micromanipulation of Ferromagnetic Upconversion Microparticles

   Presenter: Gokul Nalupurackal, IIT Madras, India

    Presentation Details   Paper

   We demonstrate the simultaneous optical trapping and magnetic manipulation of Fe-doped upconverting particles to achieve full control over six-degrees of freedom of a rigid body in optical tweezers, and the precise detection of its motion.
   
   Authors:Gokul Nalupurackal, IIT Madras / Gunaseelan M, IIT Madras / Amit Chauhan, IIT Madras / B.R.K Nanda, IIT Madras / Basudev Roy, IIT Madras

4. 17:30 - 18:00

   (UTC - 07:00)
   AW4D.4

   Second Harmonic Generation From Nanoparticles : From Free Liquid Suspensions to Controlled Single Nanoparticles

   Presenter: Pierre-Francois Brevet, Université Claude Bernard Lyon 1, France

    Presentation Details   Paper

   Nanoparticles present advantages in nonlinear microscopy, second harmonic generation (SHG) notably. They require specific design of their optical properties and spatial manipulation. We show combination of SHG microscopy and optical tweezers to this end.
   
   Authors:Zacharie Behel, Université Claude Bernard Lyon 1 / Rachael Taitt, Ecole Centrale Lyon / Yann Chevolot, Ecole Centrale Lyon / Virginie Monnier, Ecole Centrale Lyon / Yannick Mugnier, Université Savoie Mont-Blanc / Ronan Le Dantec, Université Savoie Mont-Blanc / Pierre-Francois Brevet, Université Claude Bernard Lyon 1

   Invited

New Techniques for in vivo Microscopy

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1. 16:30 - 17:00

   (UTC - 07:00)
   BW4B.2

   Methods for Imaging Synaptic Activity in Vivo

   Presenter: Kaspar Podgorski, Allen Institute for Brain Science, United States

    Presentation Details

   Abstract not available.
   
   Authors:Kaspar Podgorski, Allen Institute for Brain Science

   Invited In Person

2. 17:00 - 17:15

   (UTC - 07:00)
   BW4B.3

   Synaptic Resolution Two-Photon Stimulation and Imaging of Neural Activity With Bessel Beam Light-Sheet Microscopy

   Presenter: Dongli Xu, The University of Arizona, United States

    Presentation Details   Paper

   We report combining two-photon Bessel beam light-sheet microscopy with two-photon uncaging to perform all-optical neural stimulation and imaging at synaptic resolution. Imaging results from mouse brain slices under 2P uncaging of glutamate will be presented.
   
   Authors:Dongli Xu, The University of Arizona / Jun Ding, Stanford University / Leilei Peng, The University of Arizona

   In Person

3. 17:15 - 17:30

   (UTC - 07:00)
   BW4B.4

   Aberration Correction in Long GRIN-Based Microendoscopes Using 3D Printed Polymer Lenses

   Presenter: Chiara Nardin, Istituto Italiano di Tecnologia, Italy

    Presentation Details   Paper

   We compensated optical aberrations in 6.4 mm-long and 8.8 mm-long GRIN-based endoscopes using 3D microprinted polymer lenses. Corrected microendoscopes exhibit improved axial resolution and extended field-of-view in two-photon fluorescence imaging experiments.
   
   Authors:Chiara Nardin, Istituto Italiano di Tecnologia / Andrea Sattin, Istituto Italiano di Tecnologia / Innem Reddy, King Abdullah University of Science and Technology / Carlo Liberale, King Abdullah University of Science and Technology / Tommaso Fellin, Istituto Italiano di Tecnologia

   In Person

4. 17:30 - 18:00

   (UTC - 07:00)
   BW4B.1

   Customized Optical Microscopy and Computational Algorithms for Brain Imaging and Optogenetic Stimulation

   Presenter: Yi Xue, University of California Davis, United States

    Presentation Details

   We developed a one-photon microscope to photo-stimulate and image multiple neurons in the mouse brain by controlling light field in 4D.
   
   Authors:Yi Xue, University of California Davis

   Invited Virtual

5. 18:00 - 18:30

   (UTC - 07:00)
   BW4B.5

   Technology Development for Understanding of Neuronal Circuitry in the Central Nervous System

   Presenter: Yasaman Soudagar, Neurescence Inc., Canada

    Presentation Details

   Abstract not available.
   
   Authors:Yasaman Soudagar, Neurescence Inc.

   Invited In Person

Spectroscopy

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1. 16:30 - 17:00

   (UTC - 07:00)
   DW4A.1

   Raman-Based Spectroscopic Tools for Characterizing Pediatric Eosinophilic Esophagitis

   Presenter: Andrea Locke, Vanderbilt University, United States

    Presentation Details

   Eosinophilic Esophagitis is a chronic inflammatory esophageal disease. The clinical gold-standard relies on repeat histological assessment of biopsied tissues. Raman spectroscopy has the potential for identifying key biochemical changes, in-situ, indicative of the disease activity.
   
   Authors:Andrea Locke, Vanderbilt University / Ezekiel Haugen, Vanderbilt University / Emma Bartelsen, Vanderbilt University / Regina Tyree, Vanderbilt University Medical Center / Hernan Correa, Vanderbilt University Medical Center / Anita Mahadevan-Jansen, Vanderbilt University / Girish Hiremath, Vanderbilt University Medical Center

   Invited Virtual

2. 17:00 - 17:15

   (UTC - 07:00)
   DW4A.2

   Raman Character of the Photosensitizer-Free Singlet Oxygen Photogeneration Within Water

   Presenter: Aristides Marcano Olaizola, Delaware State University, United States

    Presentation Details   Paper

   We study the Raman photogeneration of singlet oxygen in water without using photosensitizers. We demonstrate the Raman character of the effect by measuring the signal’s frequency shift, time evolution, power dependence, and ring structure.
   
   Authors:Aristides Marcano Olaizola, Delaware State University / Ameen Zerrad, Delaware State University / David KIngsley, USDA

   In Person

3. 17:15 - 17:30

   (UTC - 07:00)
   DW4A.3

   Numerical Ray Optics Analysis of Probe Optics Prototype for Mobile-Based Raman Spectrometer

   Presenter: Uttam Pal, IIITDM Kancheepuram, India

    Presentation Details   Paper

   This work involves the numerical ray optics simulation of the probe optics prototype for a mobile camera-based Raman spectrometer toward cancer diagnosis using core biopsy tissues
   
   Authors:Sabrivel R, Sathyabama Institute of Science and Technology / Sollen Selvan V, Sathyabama Institute of Science and Technology / Naveen Sanjay, SRM Institute of Science and Technology / Uttam Pal, IIITDM Kancheepuram

   Virtual

4. 17:30 - 17:45

   (UTC - 07:00)
   DW4A.4

   Analysis of Non-Alcoholic Fatty Liver Through Intensity Maps Applying Raman Spectroscopy

   Presenter: Elizabeth Ramirez, UASLP, Mexico

    Presentation Details   Paper

   Steatosis is when the liver accumulates more than 5% of its total weight in fat. Raman imaging was used to evaluate the molecular content of ex vivo rat liver tissue samples, where proteins and fatty acids were found in abundance.
   
   Authors:Elizabeth Ramirez, UASLP / Edgar Guevara, UASLP / Guadalupe Donjuan-Loredo, UASLP / Ricardo Espinosa-Tanguma, UASLP / Roberto Salgado-Delgado, UASLP / Miguel Ramírez-Elías, UASLP

   In Person

5. 17:45 - 18:00

   (UTC - 07:00)
   DW4A.5

   Parallel Interferometric Near-Infrared Spectroscopy Monitors the Blood Flow and Absorption of the Human Brain in Vivo

   Presenter: Dawid Borycki, Polish Academy of Sciences, Poland

    Presentation Details   Paper

   We developed and applied parallel interferometric near-infrared spectroscopy (πNIRS) for rapid cerebral blood flow and absorption monitoring. By operating ~100-500x faster than competing optical techniques, we can monitor prefrontal cortex activation in humans in vivo
   
   Authors:Dawid Borycki, Polish Academy of Sciences / Saeed Samaei, Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences / Klaudia Nowacka, Polish Academy of Sciences

   In Person

6. 18:00 - 18:15

   (UTC - 07:00)
   DW4A.6

   Transabdominal Fetal Signals Measurement Using Fiber-Based Frequency-Modulated Continuous-Wave Near-Infrared Spectroscopy in a Fetal Lamb Model

   Presenter: Shing-Jiuan Liu, University of California, Davis, United States

    Presentation Details   Paper

   We propose a novel transabdominal fetal pulse oximetry using frequency-modulated continuous-wave near-infrared spectroscopy. By measuring the time-resolved reflectance of light shined on pregnant sheep’s abdomen, we experimentally recovered the fetal signals originating from deep tissue.
   
   Authors:Shing-Jiuan Liu, University of California, Davis / Su Yeon Lee, University of California, Davis / Christopher Pivetti, University of California, Davis / Edwin Kulubya, University of California, Davis / Aijun Wang, University of California, Davis / Diana Farmer, University of California, Davis / Soheil Ghiasi, University of California, Davis / Weijian Yang, University of California, Davis

   Student Paper Finalist In Person

7. 18:15 - 18:30

   (UTC - 07:00)
   DW4A.7

   Dual-Wavelength Interferometric Near-Infrared Spectroscopy (INIRS)

   Presenter: Dibbyan Mazumder, NYU Langone Health, United States

    Presentation Details   Paper

   We demonstrate an interferometric near-infrared spectroscopy (iNIRS) instrument, operating simultaneously at two wavelengths, for quantification of optical properties of turbid media. Measurements are demonstrated on a fluid phantom.
   
   Authors:Dibbyan Mazumder, NYU Langone Health / Oybek Kholiqov, University of California Davis / Vivek Srinivasan, NYU Langone Health

   Virtual

Reporters and Contrast Agents - Nanoparticles and Photodynamic Therapy

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1. 16:30 - 17:00

   (UTC - 07:00)
   OW4E.1

   Upconverting Nanoparticles for High-Contrast Breast Cancer Immune-Histochemistry Biomarker Mapping

   Presenter: Stefan Andersson Engels, Tyndall National Institute, Ireland

    Presentation Details   Paper

   The use of UCNP staining to distinguish different levels of HER2 expression in HER2 analyte control and HER2-positive breast cancer tissue biopsies provided an improved contrast - 25 times as compared to conventional DAB staining.
   
   Authors:Stefan Andersson Engels, Tyndall National Institute / Sanathana Konugolu Venkata Sekar, Tyndall National Institute / Hui Ma, Tyndall National Institute / Katarzyna Komolibus, Tyndall National Institute / Gokhan Dumlupinar, Tyndall National Institute / Krzysztof Krawczyk, Lumito AB / Matthias Mickert, Lumito AB

   Invited In Person

2. 17:00 - 17:30

   (UTC - 07:00)
   OW4E.3

   Evaluating the Imaging and Therapeutic Performance of a Dual Function Antibody Conjugate in Head and Neck Cancer Spheroids

   Presenter: Mohammad Saad, Massachusetts General Hospital and Harvard Medical School, United States

    Presentation Details   Paper

   This study demonstrates the efficacy of a molecular-targeted dual function antibodybased probe in detecting and treating microscopic oral cancer spheroids by a combination of fluorescence and photoacoustic imaging and photoimmunotherapy.
   
   Authors:Mohammad Saad, Massachusetts General Hospital and Harvard Medical School / Stacey Grimaldo-Garcia, Middlebury College / Leslie Contreras, Northeastern University / Allison Sweeney, Tufts University / Scott Selfridge, Akita Innovations / Robert Pawle, Akita Innovations / Srivalleesha Mallidi, Massachusetts General Hospital and Harvard Medical School / Tayyaba Hasan, Massachusetts General Hospital and Harvard Medical School

   Invited Virtual

3. 17:30 - 17:45

   (UTC - 07:00)
   OW4E.4

   Magnetic Gold Nanorods for Contrast in Magnetomotive Optical Coherence Tomography (OCT)

   Presenter: Amy Oldenburg, Univ of North Carolina at Chapel Hill, United States

    Presentation Details   Paper

   We propose plamonic gold nanorods decorated with magnetic iron oxides for modulating polarized OCT signals. We show that signal modulation depends upon the direction of the applied magnetic field consistent with a magnetic trapping model.
   
   Authors:Amy Oldenburg, Univ of North Carolina at Chapel Hill / Mehedi Rizvi, North Carolina State University / Joseph Tracy, North Carolina State University

   In Person

4. 17:45 - 18:00

   (UTC - 07:00)
   OW4E.5

   Synthetic Starch-Like Particles for Second Harmonic Generation Microscopy

   Presenter: Richard Cisek, Saint Mary's University, Canada

    Presentation Details   Paper

   A starch granule model system of maltodextrin spherulites was investigated with polarization-resolved second harmonic generation, revealing similar macroscale arrangement, higher intensity, and the presence of highly aligned microdomains, similar to native starch granules.
   
   Authors:Richard Cisek, Saint Mary's University / Elisha Bennett, Saint Mary's University / MacAulay Harvey, Saint Mary's University / Danielle Tokarz, Saint Mary's University

   In Person

5. 18:00 - 18:30

   (UTC - 07:00)
   OW4E.2

   Photoacoustic Imaging

   Presenter: Srivalleesha Mallidi, Tufts University, United States

    Presentation Details

   Abstract not available.
   
   Authors:Srivalleesha Mallidi, Tufts University

   Invited In Person

Multiphoton Techniques

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1. 16:30 - 17:00

   (UTC - 07:00)
   NW4C.1

   An Energetic, Tunable, Ultra-Broadband Fiber Source for Imaging and Spectroscopy

   Presenter: Sixian You, Massachusetts Institute of Technology, United States

    Presentation Details

   We propose a new way of controlling nonlinear effects in multimode fibers with a programmable fiber shaper, providing unparalleled access to spatiotemporal degrees of freedom for high-peak-power two-octave femtosecond tunable fiber sources.
   
   Authors:Sixian You, Massachusetts Institute of Technology

   Invited Virtual

2. 17:00 - 17:30

   (UTC - 07:00)
   NW4C.2

   Rapid Large-Volume Tissue Imaging With Multiplane Parallel Strip-Scanning Two-Photon Microscopy

   Presenter: Vincent Ralph Ching-Roa, University of Rochester, United States

    Presentation Details   Paper

   We present a parallel point scanning two-photon microscope using silicon photomultiplier arrays, enabling imaging up to 200 MP/s while retaining high light throughput using nondescanned detection. We demonstrate resilience against scattering by imaging skin specimens.
   
   Authors:Vincent Ralph Ching-Roa, University of Rochester / Chi Huang, University of Rochester / Michael Giacomelli, University of Rochester

   Invited In Person

3. 17:30 - 17:45

   (UTC - 07:00)
   NW4C.3

   Intravital Multiphoton Microscopy of Human Skin With Label-Free Molecular Contrast

   Presenter: Mihaela Balu, UC Irvine Beckman Laser Institute, United States

    Presentation Details   Paper

   This presentation will highlight the most recent results of exploring the feasibility of a custom designed clinical multiphoton imaging platform to identify and distinguish immune cell populations in human skin, based on label-free molecular contrast.
   
   Authors:Amanda Durkin, UC Irvine Beckman Laser Institute / Juvinch Vicente, UC Irvine Beckman Laser Institute / Katelyn Dilley, UC Irvine Beckman Laser Institute / Moran Lemberger, Kaplan Medical Center / Anand Ganesan, University of California, Irvine / Mihaela Balu, UC Irvine Beckman Laser Institute

   In Person

4. 17:45 - 18:15

   (UTC - 07:00)
   NW4C.4

   Speeding in Microscopy: From Optofluidic Single-Cell Imaging to in-Vivo Animal Imaging

   Presenter: Kevin Tsia, University of Hong Kong, Hong Kong

    Presentation Details

   This talk will discuss how the recent advances in scaling the speed of different imaging modalities (from quantitative phase, light-sheet to kHz multiphoton imaging) could broadly accelerate biomedical discovery/applications (e.g. neuroscience and single-cell analysis).
   
   Authors:Kevin Tsia, University of Hong Kong

   Invited In Person

5. 18:15 - 18:45

   (UTC - 07:00)
   NW4C.5

   Developing High Confinement and Low-Loss Silicon Nitride Photonic Devices for Optical Coherence Tomography

   Presenter: Xingchen Ji, Shanghai Jiao Tong University, China

    Presentation Details   Paper

   There is a surging interest in developing integrated Optical Coherence Tomography (OCT) system. However, most components are based on silicon which cannot be used for wavelength below 1.2 µm. Here, we discuss the potential of using Si₃N₄ photonic devices for OCT.
   
   Xingchen Ji is a 2022 Optica Foundation Challenge Winner optica.org/FoundationChallenge.
   
   Authors:Xingchen Ji, Shanghai Jiao Tong University

   Invited Virtual