The Phasor Plots: A Tool for Democratize the Access and Use of Time-Resolved and Hyperspectral Imagi
This webinar is hosted By: Microscopy and Optical Coherence Tomography Technical Group
22 July 2022 11:00 - 12:00
Eastern Daylight/Summer Time (US & Canada) (UTC -04:00)The phasor plot approach is a model-free method to analyze and interpret time-resolved and hyperspectral imaging. Traditionally, the analysis of time-resolved or hyperspectral imaging requires a data fitting or demixing model. The model selection requires specific knowledge, which restricts its adoption of the need for expertise to succeed in the data analysis and interpretation. Due to the model-free fashion, intuitiveness, and simple rules, the phasor plots are democratizing the use of these advanced imaging technologies. In this webinar, Dr. Malacrida will cover the fundamentals and their applications for addressing different biophysical problems straightforward. For instance, the role of water dynamics and molecular crowding in cells and animals will be elucidated by the phasor approach in combination with dimethylamino-naphthalene probes or the harmonic content for the hyperspectral unmixing of many components. Finally, Dr. Malacrida will share the technological developments at the Advanced Bioimaging Unit (UBA) and the many opportunities for regional students and core facility staff to research and visit the UBA.
Subject Matter Level: Intermediate - Assumes basic knowledge of the topic
What You Will Learn:
• Fundamentals of hyperspectral and time-resolved imaging.
• Principles and application of the phasor plots.
• Applications of phasor plots to biological-driven questions.
Who Should Attend:
• Scientists interested in understanding fundamentals of time-resolved and hyperspectral imaging.
• Researchers interested in adopting phasor plots for time-resolved and hyperspectral imaging.
About the Presenter: Leonel Malacrida, Advanced Bioimaging Unit, Universidad de la Republica & Institut Pasteur Montevideo, Uruguay
Leonel Malacrida’s journey to advanced bioimaging started during his Ph.D. research in biophysics in Uruguay. He first studied lung surfactant —a biofilm coating the lung interior that enables us to breathe— and wanted to understand this biological material at the molecular level. During Malacrida's postdoc at the Laboratory for Fluorescence Dynamics at the University of California, Irvine, he developed into opportunities using advanced microscopy to evaluate biology quantitatively. Malacrida developed several methods and instrumentations into the advanced microscopies, such as the Multidimensional Phasor approach, connectivity maps, and sideSPIM. Leonel is particularly interested in developing methods to image cells hidden in deep layers of tissues not accessible to regular microscopes. As an Associate Professor in the School of Medicine at the Universidad de la República of Uruguay, he recently established the Advanced Bioimaging Unit.
